Sequential Transfer

A policy of sequential or consecutive transfer on Day 3 and Day 5 embryos in patients with adequate number of retrieved oocytes ,yields the advantages of a good embryo implantation rate, satisfactory pregnancy rate and an acceptable multiple pregnancy rate at the same time avoiding complications of blastocyst transfer like cancellation of the transfer cycle and higher multiple order births. However, the results of this approach depend on the number of fertilized oocytes and the quality of the fertilized embryos. Thus, sequential transfer approach has the advantage of blastocyst transfer without exposing the whole cycle to the risk of cancellation.

Under the assumption that embryos that cease to develop in utero, result in implantation failure, growing them to the blastocyst stage will serve two goals. First, it will enable better selection of embryos for transfer and second it will promote more physiologic synchronization with the endometrium and capability of achieving the “implantation window”.

 However, it is possible that no blastocyst forms during culture with the risk of cycle cancellation. So this is an approach that is only suitable for patients who produce many embryos. The rationale behind sequential embryo transfer is that, during the first day-3 transfer, the embryos (1 or 2 embryos) may induce an increase in endometrial receptivity, thereby creating a better endometrial environment for the second transfer (1 blastocyst) on day 5. Our unit is specialised in this technique. We get many patients from all over India and world, who have failed multiple times outside with other doctors . We use this technique and achieve pregnancies in most of them.

Sequential embryo transfer involves transfer of cleavage(day 3) and blastocyst (day 5) The preparation for transfer in frozen cycles will start from day 2 or day 3 of period. Your lining and ovaries will be evaluated using a transvaginal ultrasound

Step-by-Step Procedure of Sequential Transfer

1
Our team of experts will evaluate any other medical factors and start with medications that will help the lining to grow.
2
The lining will be assessed for thickness, blood flow to optimize the success of embryo transfer
3
Once the lining has grown satisfactorily, progesterone will be added to mimic the normal body changes and make the lining receptive for the embryo to implant
4
The embryo transfer will be done twice in the same cycle under ultrasound guidance to deposit the best selected embryos at the correct location within the uterine cavity