Blastocyst Culture And Transfer

Blastocyst culture (BC)and transfer is a technique developed for in vitro fertilization (IVF). This procedure intends to maximize pregnancy rates while minimizing the risk of multiple pregnancies. Embryos are typically cultured for 3 days (cleavage stage with 8-10 cells) before being transferred into the uterus. By extending the culture to 5 or 6 days (blastocyst culture), some embryos will develop to the blastocyst stage (large embryos of 150 cells with a cavity and a cover called trophectoderm and zona).

This allows the embryologist to select more advanced embryos with better potential for implantation at the time of the transfer. Since the implantation rate per embryo is significantly better with blastocyst transfer, one can afford to transfer only one embryo or at the most two embryos and attain satisfactory clinical pregnancy and live birth rates, at the same time avoiding complications of multiple gestation. Blastocyst stage transfer is more physiological, as the endometrium is synchronized with the developmental stage of the embryo. Blastocyst transfer yields a better clinical pregnancy as well as live birth rate, if one considers pregnancy rate per transfer attempt. Another major advantage of blastocyst transfer is its role in patients with previous multiple failed attempts at IVF.In our unit we offer single blastocyst transfer in the first attempt. It is better to freeze all embryos and transfer the embryo in the next cycle, also called frozen thaw cycle. Just prior to freezing, the blastocyst can be genetically analysed using the technique of PGT A (preimplantation genetic testing for aneuploidy). In this the embryo is biopsied. The 3-4 cells of biopsy are sent for genetic analysis. We have our own in-house genetictesting using the S5 Ian Torrent Next Generation Sequencing. The blastocysts are frozen after biopsy, in single individual straws, which are numbered. The report of NGS comes in 1 -2 months. After two months we can transfer a single euploid (genetically normal) blastocyst and obtain very high pregnancy rate, with singleton pregnancy and low miscarriage rate.

Patients who require pre-implantation genetic diagnosis for genetic disorders like thalassemia, sickle cell anaemia, balanced translocation, spinal muscular dystrophy and fragile x syndrome will also need to undergo trophectoderm biopsy, freezing of Blastocyst, testing the cells using NGS and transferring normal embryo in subsequent embryo Transfer cycles. we already have helped few patients of thalassemia and sickle cell anaemia to have normal babies following PGT M.

Step-by-Step Procedure of simply dummy text

1
simply dummy text
2
simply dummy text
3
simply dummy text
4
simply dummy text
5
simply dummy text
6
simply dummy text

Common Questions

If you could not find an answer to your query, please contact us.

Blastocyst is a day 5 embryo and has typically between 150-200 cells. It has a fluid filled cavity called as blastocoele and two distict types of cells called as inner cell mass which later becomes fetus and an outer cell mass which develops in to placenta.
a. They have a higher surivival rate when frozen and thawed.
b. Single blastocyst transfer can avoid twin pregnancy.
c. Biopsy of the embryo for pre implantation genetics is done on the outer cell mass of the blastocyst.